Question : Flowers! Orchids...?
I heard that Orchids can sterilize...? What??? Does anybody know about that. I heard It on Tv but missed the last part of it.
- asked by NYLA
All Answers:
Answer #1
No never heard of anything like that.... but how would they anws? just by having
them in the room ? or what? thats strange !
- answered by ☆time to wonder☆
Answer #2
To avoid bacterial and fungal growth, which is detrimental to culture growth,
explants are surface-sterilized before they are used to establish in vitro
axenic cultures. The most common disinfectants are listed below with the
concentration and exposure times that preserve the explant, but at the same time
destroy any microbial contamination.Orchid Seed Sterilization Orchid seeds
are very small and contain little to no food reserves. A single seed capsule may
contain 1,500 to 3,000,000 seeds. Sowing the seed in vitro makes it possible to
germinate immature seed (green pods). It is much easier to sterilize green
capsules than individual seeds after the capsule has split open. Lucke (1971)
indicated that orchid seed can be sterilized when the capsule is about
two-thirds ripe. Listed below are the estimated normal ripening times of
capsules for various orchid speciesGreen capsule sterilization:Soak the capsule
in a 100% bleach solution for 30 minutes. Dip the capsule into 95% alcohol, and
flame. Under aseptic conditions, open the capsule and scrape out the seed.
Carefully layer the seed over the surface of the culture medium. Dry seed
sterilization:Collect seed and place in either a small flask or bottle, or place
in a shortened pipet which has one end sealed with cotton. Seal the other end of
the pipet with cotton, once the seed has been placed in the pipet. Prepare a
solution containing 5-10% commercial bleach containing a few drops of Tween 20.
Add the bleach solution to the flask, or draw up the solution into the pipet.
Swirl the flask containing the seed and bleach or repeatedly draw and aspirate
the bleach solution in and out of the pipet. Sterilize the seed for 5-10
minutes. Remove the bleach solution and rinse the seed with sterile tissue
culture grade water. Transfer the seed to sterile culture medium.
- answered by babitha t
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